Main field of study: 011200 Physics
Area of specialization: Molecular Biophysics
Department of Molecular Biophysics
Supervisor: Prof. Dr. N.A. Kasyanenko
Referee: ass. of Prof. S.V. Paston 

Study of DNA complexes with biologically active compounds in solutions
G.V.Alekseev

DNA interaction  in the solution with diphthalocyanine (tetraazobenzoporfirinami) coordination compounds of Cu, Co, Ni with "sandwich" structure (CuPc, CoPc, NiPc) was investigated. The effect of the light-sensitive surfactants based on azobenzene on DNA binding with CuPc, CoPc, NiPc was considered. The presence of DNA in the aqueous-salt solution of CuPc, CoPc, NiPc  stabilizes the system and prevents precipitation of drugs. It is shown that the CuPc, CoPc, NiPc interact with phosphate groups and does not affect the secondary structure of macromolecule, but causes a decrease in its volume. The state of CuPc, CoPc, NiPc during the  binding with DNA molecule in a solution differs slightly (depending on the type of metal), as evidenced by the variation of the absorption spectra. It is shown that pre-irradiation of  surfactant by UV in the solution before binding to DNA  leads to the same result as UV irradiation of already formed complexes. The experiment showed the fundamental ability to create multi-component systems of DNA-surfactant-metal complexes. In the formation of the ternary systems of DNA-surfactant-metal complexes the order of addition of the components into DNA solution does not matter. Metal complexes bind to DNA in the presence of surfactant and DMSO, and surfactant interaction with DNA is realized after DNA-CuPc complex formation. 

The list of the publications
1. N. Kasyanenko, L. Lysyakova, Z. Qiushi, G. Alexeev, Z. Reveguk.  Gene vectors with the inclusion of anticancer drugs and metal nanoparticles. In: Book of Abstracts of  9th European Biophysics Congress, 2013, Lisboa, Portugal, P-297, № 253