Author: Михайлов

Main field of study: 011200 Physics
Area of specialization: 09 Molecular Biophysics
Department of Molecular Biophysics and Polymer Physics
Scientific supervisor: PhD, Associate Professor A.M. Polyanichko
Reviewer: PhD V.B. Rogozhin

Mechanisms of formation of intermolecular protein complexes on the example of BSA, H1 and HMGB1 proteins
N.V. Mikhailov

Histone H1 and non-histone HMGB1 protein are the most abundant proteins in chromatin. The main function of H1 and HMGB1 proteins is the regulation of DNA compaction and hence regulation of genes expression. 
This work is devoted to the investigation of the mechanisms of formation of intermolecular protein complexes. BSA solutions were used as model systems for working out the best conditions of experimental methods.
Mechanisms of protein complexes formation were investigated by the methods of Analytical Ultracentrifugation, UV-spectroscopy, Dynamic Light Scattering, measuring of Electrical Double Layer, Polyacrylamide Gel Electrophoresis and Gel-Filtration.

The list of the publications
1. T.Yu. Starkova, N.V. Mikhailov, E.I. Kostyleva, E.V. Chikhirzhina, A.M. Polyanichko. Interaction between linker histone H1 and non-histone protein HMGB1 in vitro// Book of abstract, 38^th FEBS CONGRESS “Mechanisms in Biology”. Russia, 2013. P. 129.
2. E.V. Chikhirzhina, E.I. Kostyleva, N.V. Mikhailov, A.M. Polyanichko. The investigation of the structure of supramolecular complexes between DNA and “linker” chromatin proteins using spectroscopic approaches// Book of abstract, The 15^th  European Conference on the Spectroscopy of Biological Molecules. UK, 2013. P. 34 – 35.